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Organ:
lung Disease:
adenocarcinoma Derived from metastatic
site: pleural effusion
Permits/Forms:
In addition to the MTA
mentioned above, other ATCC and/or regulatory
permits may be required for the transfer of this
ATCC material. Anyone purchasing ATCC material is
ultimately responsible for obtaining the permits.
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regarding the specific requirements for shipment
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The cells are distributed for research purposes only. The
Memorial Sloan-Kettering Cancer Center releases the line
subject to the following: 1.) The cells or their products must
not be distributed to third parties. Commercial interests are
the exclusive property of Memorial Sloan-Kettering Cancer
Center. 2.) Any proposed commercial use of these cells must
first be negotiated with The Director, Office of Industrial
Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212)
717-3439.
Tumorigenic:
Yes, forms well differentiated grade I adenocarcinoma in
nude mice
The stemline chromosome number is hypotriploid with the 2S
component occurring at 1.4%. Approximately 20 markers were
common to most S metaphases, of which i (1p), t(12;?) and
t(18;?) were generally paired; t(6t?) had an HSR segment of q
arm, and M7 had two secondary constrictions. Normal
chromosomes 1, 13, 15 and 17 were absent, and the X was
disomic. No Y chromosome was detected in the QM stained
preparations.
The patient had received prior therapy with cytoxan,
bleomycin and adriamycin.
Propagation:
ATCC complete growth medium: Minimum essential
medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted
to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential
amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine
serum, 10% Temperature: 37.0C Atmosphere:
air, 95%; carbon dioxide (CO2), 5%
Subculturing:
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-
0.53 mM EDTA solution to remove all traces of serum that
contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and
observe cells under an inverted microscope until cell layer
is dispersed (usually within 5 to 15 minutes). Note: To
avoid clumping do not agitate the cells by hitting or
shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to
facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate
cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new
culture vessels.
Incubate cultures at
37°C.
Subcultivation ratio: A
subcultivation ratio of 1:3 to 1:6 is
recommended
Medium renewal: 2 to 3 times per
week
Preservation:
Freeze medium: Complete growth medium supplemented
with 5% (v/v) DMSO Storage temperature: liquid
nitrogen vapor phase
Related Products:
Recommended medium (without the additional supplements or
serum described under ATCC Medium): ATCC 30-2003 recommended
serum: ATCC 30-2020
References:
21869: Fogh
, editor. Human tumor cells in vitro. New York: Plenum Press;
1975, pp. 115-159. 22536: Fogh J , et al. Absence of HeLa
cell contamination in 169 cell lines derived from human
tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J , et al. One hundred and
twenty-seven cultured human tumor cell lines producing tumors
in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed:
327080 24381: Fogh J . Cultivation,
characterization, and identification of human tumor cells with
emphasis on kidney, testis, and bladder tumors. Natl. Cancer
Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
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